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Hibiscus pollen


Guest rubidium

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Guest rubidium

Hi Al.

This image was taken with the R9 and DMR attached to a circa-1965 Leitz Orthoplan microscope. The photo below shows the lens used - a Leitz 6.3x Fluotar, meaning that it contains fluorite glass elements (10 elements in 7 groups) that give nearly, but not quite, the performance of an apochromat. Illumination is from below using an arrangement referred to as "darkfield", meaning that a stop blocks the specimen illumination directly along the optical axis but permits it in an annular zone surrounding the specimen - a contrast enhancing technique. The total magnification, including the DMR's 1.37 crop factor, is about 250x, so that the large pollen grain to the left of center is about 70 microns in diameter.

Cheers,

Jim

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